RP-HPLC Purity Testing Method
Reversed-phase high-performance liquid chromatography (RP-HPLC) is the standard method for peptide purity determination. The peptide sample is injected onto a C18 reversed-phase column and eluted with an acetonitrile/water gradient containing 0.1% trifluoroacetic acid (TFA). The UV detector (typically at 214nm or 220nm) records the absorbance of each eluting component.
Purity is calculated as the area percentage of the main peptide peak relative to the total integrated peak area. A purity of ≥98% means that at least 98% of the UV-absorbing material in the sample is the target peptide.
ShiLai™ Purity Standards
| Parameter | Standard |
|---|---|
| Purity method | RP-HPLC (C18 column, UV 214nm) |
| Standard specification | ≥98% by RP-HPLC |
| Testing frequency | Every batch before release |
| COA documentation | Full chromatogram + peak integration table |
| Identity confirmation | LC-MS (every batch) |
Request a Sample COA
Contact ShiLai™ Healthcare to request a sample COA with RP-HPLC purity data and LC-MS identity confirmation.
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